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Research Paper Open Access
The effects of exogenous supply of precursors, such as cholesterol, pregnenolone, L-ascorbic acid, shikimic acid, D-limonene, and squalene (50mg/1) on production of capsaicin in static cultures were observed. There were appreciable changes in capsaicin content due to treatment of 50 mg/1 of D-limonene (0.136 percent mg of capsaicin) and 100 mg/1 of L-ascorbic acid (0.169 mg of capsaicin). The capsaicin was detested only in the medium indicating its extracellular in-vitro accumulation. The precursors such as squalene, pregnenolone, and shikimic acid did not alter significantly the bioproduction pattern of capsaicin.