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Eight different endophytic fungus was isolated and identified from the various parts of Viscum album, lectin was isolated from all the endophytic fungal sample and host Viscum album. Lectin isolated was blood group specific lectin agglutinating A+ve erythrocytes. Carbohydrate specificity of lectin was varied, Aspergillus flavus, Fusarium moniliforme, Fusarium oxysporum, Trichothecium sp, and Viscum album lectin was D-galactose and N-acetyl glucosamine specific lectin, Alternaria sp and Pencillium sp lectin was fructose specific lectin, Aspergillus niger and Cladosporium sp was Maltose and D-galactose specific lectin. Biochemical characterization of lectin showed that metal chelating agent EDTA has no effect on the hemaglutinating activity of Viscum album, Aspergillus flavus, Alternearia sp, Fusarium oxysporum, Trichothecium sp, lectin but EDTA inhibited the hemaglutinating activity of Pencillium sp, Cladosporium sp, Aspergillus niger lectin, activity was restored by adding divalent cations Mg2+, Mn2+. pH sensitive profile shows that lectin of Viscum album and its all endophytic fungal lectin retained the hemagglutinating activity with in the pH range 6-9, Viscum album and Aspergillus niger, Cladosporium sp, Fusarium moniliforme lectin was thermostable up to 600C, Aspergillus flavus ,Alternearia sp, Fusarium oxysporum, Trichothecium sp lectin was stable upto 500C. SDS PAGE of Aspergillus flavus, Fusarium moniliforme, Fusarium oxysporum, Trichothecium sp, D-galactose and N-acetyl glucosamine specific lectin showed the presence of 64kD protein similar to Viscum album lectin, PAS staining assay confirmed the presence of lectin in all endophytic fungal samples.
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Author(s): Sadananda TS Govindappa M Ramachandra YL
Lectin, endophytes, Viscum album, Hemagglutination, PAS staining