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To investigate the sublethal effects of pesticide lambda cyhalothrin on biochemical and the liver histology of Oreochromis mossambicus under long term exposure. The toxicity tests were conducted following method of Finney (1964). Based on the acute toxicity assay the fish were exposed to 0.0025 ppm sublethal concentration. The glycogen content in liver tissue and glucose content in blood were estimated at the end of 10, 20 and 30 days. The liver tissues were removed and fixed in Bouin’s fluid and then they processed for microphotograhs. Glycogen content was found to be depleted in liver at all periods after treatment. In contrast to the observation of glycogen, the glucose level was elevated in the blood at all periods. In control tilapia, the liver was comprised of polygonal hepatocytes with centrally placed nucleus. Pesticide induced dilation of blood sinusoids, vacuolization and disintegration of cell boundaries were noticed in the 10 and 20 days of exposed fish. The complete damage of hepatocytes and loss of integrity of cell wall was noticed about 30 days of exposure. From the results stated above it can be concluded that the finding of the present study indicate that histological changes observer serve as “biomarkers” for assessing pesticide toxicity in aquatic environment.
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Author(s): K MUTHUKUMARAVEL O SATHICK AND S RAVEENDRAN
Lambda cyhalothrin, toxicity, liver, histology and O. mossambicus, physiology, cell and molecular biology, genetics, biochemistry, biotechnology, bioinformatics, microbiology, immunology, parasitology