alexa Abstract | Laser Ablation Inductively Coupled Plasma Mass Spectrometry in Biomedicine and Clinical Diagnosis

Cellular & Molecular Medicine: Open access
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access

Abstract

 Background: Toxic metal accumulation in the body that may be caused by inherited disorders, chronic intake, or acute intoxication. Vice versa, inadequate daily supply with a specific trace element results in shortcomings and manifests in clinical symptoms. In diagnostics, the determination of exact metal concentrations or the measurement of proteins correlating with the concentration of a specific metal is widespread. In addition, several histopathological stains are available, allowing identification of specific metal deposits in tissue. The introduction of novel metal imaging techniques has extended the repertoire of analytical possibilities. In particular, laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS) has undergone an intensive progress in recent years. It has multi-element capability, granting the analysis of a large variety of biological materials with high spatial resolution. It further can identify and quantitate a large number of metals and metalloids at extremely low concentrations. Methods and findings: We here optimized and extended previous studies in which we used LA-ICP-MS for trace metal imaging in livers and brains of wild type and Atp7b deficient mice that represents an experimental model of human Wilson's disease. We show that the observed alterations in metal content and distribution are closely linked to the progression of the disease. While the accumulation of copper in the brain can be assigned to special regions, the overload with copper is initially distributed uniformly, while the regional distribution of individual metals is significant modulated in necrotic and tumorigenic tissue areas.

Conclusion: These illustrations show that LA-ICP-MS is a highly powerful and innovative analytical technique that will have tremendous impact on biomedical research and diagnostics of metal disorders. Abbreviations: Atp7b: gene encoding a copper-transporting P-type ATPase; EDX: Energy-Dispersive X-ray spectroscopy; LA-ICP-MS: Laser Ablation Inductively Coupled Plasma Mass Spectrometry; LM: Light Microscopic; SIMS: Secondary Ion Mass Spectrometry; SXRF: Synchrotron X-ray Fluorescence.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Ralf Weiskirchen and Ricarda Uerlings

Keywords

Nuclear Medicine, Methods in Molecular Medicine, Genetic Medicine

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

agrifoodaquavet@omicsonline.com

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

clinical_biochem@omicsonline.com

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

business@omicsonline.com

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

chemicaleng_chemistry@omicsonline.com

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

environmentalsci@omicsonline.com

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

engineering@omicsonline.com

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

generalsci_healthcare@omicsonline.com

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

genetics_molbio@omicsonline.com

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

immuno_microbio@omicsonline.com

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

omics@omicsonline.com

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

materialsci@omicsonline.com

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

mathematics_physics@omicsonline.com

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

medical@omicsonline.com

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

neuro_psychology@omicsonline.com

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

pharma@omicsonline.com

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

social_politicalsci@omicsonline.com

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version