alexa Abstract | Method development and validation of related substances in Pantoprazole Sodium by RP HPLC

Journal of Chemical and Pharmaceutical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Original Articles Open Access

Abstract

A simple, sensitive and reproducible Reverse phase high performance liquid chromatography (HPLC) coupled with a photodiode array detector; method was developed for the quantitative determination of related substances in API. Chromatographic separation was achieved on Hypersil ODS (125 X 4.0) mm, 5μm, and the gradient eluted with runtime, 50.0 min. The eluted compounds were monitored at 290 nm, the flow rate was 1.0 mL/min, and the column oven temperature was maintained at 40oC. The resolution of Pantoprazole and three impurities was greater than 2.0 for all pairs of components. The high correlation coefficient (r2> 0.9990) values indicated clear correlations between the investigated compound concentrations and their peak areas within the test ranges. The repeatability and intermediate precision, expressed by the RSD, were less than 10%. The accuracy evaluated by performing recovery studies via a spike method, was in the range 80.0-120.0%. The performance of the method was validated according to the present ICH guidelines for specificity, limit of detection, limit of quantification, linearity, accuracy, precision, ruggedness and robustness.

To read the full article Peer-reviewed Article PDF image

Author(s): Priyadarshini S Bansode Ravindra K Kamble Chetan Singh Chauhan and Sujata S Bansode

Keywords

Pantoprazole sodium, Impurity, Validation, RP HPLC

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords