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Research Article Open Access
Rosa miniature ‘Green Ice’ is one of the most important commercial crops. For study on effect of IBA and 2ip on micropropagation of Rosa miniature ‘Green Ice’ , tree levels of IBA (0, 1 and 2 mg l-1) and tree levels of 2ip (0, 1 and 2 mg l-1) in three replications were examined. Explants selected on mature leaf and after sterilization, for callus induction transport in MS medium with IBA and 2ip. After 4 week, calli transport in new same medium for shoot induction. In this study traits such as days of callus initiation, day of shoot observation, percent of callus, number of shoot per explants, shoots fresh weight, shoot length were examined. Result showed that the IBA and 2ip effect of callus and shoot traits respectively.
IBA, 2ip, MS medium