alexa Abstract | Molecular Diagnosis of Lung Cancers.

Molecular Enzymology and Drug Targets
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access


diagnostic test. Initially used as research tools the molecular diagnostic tests have been applicable in the clinical scenario.

Purpose: Current study focuses on the significant approach of detection of epidermal growth factor receptor (EGFR) mutations in exon 18-21 and anaplastic lymphoma kinase gene (ALK) rearrangement analysis using molecular methods such as pyrosequencing and florescence in-situ hybridization (FISH) on formalin fixed paraffin embedded (FFPE) from tissue biopsy and cell blocks of 267 lung cancer patients.


Methods: In this study, FISH using a break-apart probe for the ALK gene was performed on formalin fixed paraffin-embedded tissue to determine the incidence of ALK rearrangements and hybridization patterns in 267 patients with a referred diagnosis of non-small cell lung cancer. EGFR mutations detection was performed on 267 samples by using Pyrosequencing.

Results: Among the 267 patients 11 were positive for ALK-1 gene rearrangement. 260 of the 267 cases were confirmed of adenocarcinoma and 7 of the 267 cases were squamous cell carcinoma. EGFR mutations were detected in 55 patients. The most common mutations found were exon 19 (56.36%), exon 21 (27.27%), exon 20 (3.63%) and exon 18 (9.09%). The influence of gender, non-smoking, and histological type on the EGFR mutations showed increase in female group.

Conclusions: Mutation detection testing is mandatory in lung carcinomas for the diagnosis and management of NSCLC.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Kalal Iravathy Goud


EGFR mutation analysis, Molecular diagnosis, ALK gene rearrangement, Enzyme Engineering

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version