alexa Abstract | Mycobacterial secretory SEVA TB ES-31 antigen, a chymotrypsin-like serine protease with lipase activity and drug target potential

Biomedical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Abstract

Mycobacterium tuberculosis is known to secrete number of proteins which play an important role in pathogenicity and diagnosis. A diagnostically important secreted antigen, Excretory Secretory-31 (SEVA TB ES-31) protein with serine protease activity was isolated from Mycobacterium tuberculosis H37Ra culture filtrate. Serine proteases like Chymotrypsin have been reported to possess lipase activ-ity as the catalytic triad is similar in serine protease and some lipase enzymes. In this study, ES-31 showed presence of 44.5U/mg pr of lipase activity by titrimetric assay. The lipase activity was inhib-ited by lipase inhibitor, Orlistat by 100%, as well as by serine metalloprotease inhibitors, Phenyl me-thyl sulphonyl fluoride and Ethylene Diamine Tetraacetic acid by 100%. The serine protease activity of ES-31 was inhibited by Phenyl methyl sulphonyl fluoride, Ethylene Diamine Tetraacetic acid, and orlistat by 78.3%, 64.3%, 89.4% respectively. Inhibition of serine protease activity of ES-31 by lipase inhibitor and inhibition of lipase activity of ES-31 by serine protease inhibitor suggests that the cata-lytic site of ES-31 is sensitive to both types of the inhibitors and ES-31 may be a chymotrypsin-like protein with drug target potential

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Gauri Wankhade Anindita Majumdar Pranita D Kamble BC Harinath

Keywords

Mycobacterium tuberculosis H37Ra, Mycobacterial secreted serine protease, Lipase, Orlistat

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords