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The genus Euclea (Lepidoptera: Limacoididae) is newly recorded from South India by using DNA barcoding analysis. The larvae of Euclea were collected from nearby paddy field at Viralimalai, Tiruchirappalli, Tamil Nadu. The genomic DNA was isolated from the larval heamolymph and the cytochrome oxidase subunit 1 (COI) gene and was successfully amplified. The amplified PCR product was visualized by Agarose gel electrophoresis and the size was found to be approximately 500 bp. Further, the amplified product of the COI gene was purified and sequenced. The sequence analysis revealed that the Euclea larvae collected from the study site are closely related to Euclea norba. The phylogenetic analysis provides the data to understand the distribution of this species in India and reveals the phylogeny of this genus Euclea.
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Author(s): M Kannan S Anbalagan M Krishnan K Muthukrishnan V Gokula
Heamolymph, Genomic DNA, Euclea norba, Cytochrome oxidase subunit-1, Polymerase Chain Reaction., physiology, cell and molecular biology, genetics, biochemistry, biotechnology, bioinformatics, microbiology, immunology, parasitology