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Research Paper Open Access
Three Simple and sensitive spectrophotometric methods for the determination of Pyrithioxine (PYT) are described. The first method (Method A, Î»max : 470 nm) is based on the oxidative coupling reaction of PYT with 4-Aminophenazone (4AP). The second one (Method B, Î»max : 495 nm) depends on the formation of ion-association complex of PYT with Tropaeolin OOO (TPOOO) which is extracted into chloroform. The third one (Method C, Î»max:600nm) is based on the reduction of fe3+ to fe2+ by the PYT, which forms the coloured complex with 2,4,6-tripyridyl-s-triazine (TPTZ). These methods are extended to the analysis of pharmaceutical formulations and results compared with the U.V. reference method.
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Author(s): C S P Sastry D Murali Krishna