alexa Abstract | Next Generation Sequencing In Clinical Molecular Diagnosis of Cancer: A Journey towards Personalized Diagnosis and Therapeutics

Journal of Medical Oncology and Therapeutics
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Review Article Open Access

Abstract

Most cancers are genetically complex and involve a series of patho-genomic DNA mutations or genetic hits. The order of development of these genetic hits is extremely diverse and this diversity in order of development of genomic mutations makes the diagnosis of most types of cancers very much challenging. So, each cancer needs to be treated as a single novel case. So, a new era has arrived when personalized diagnosis of cancer can be offered with development of massively parallel sequencing technology, popularly known as Next- Generation Sequencing. With this technology, it is quite possible to look into the substantial part of the genome of a cancer cell within a very short period of time and at an affordable cost.

Next Generation sequencing represents an effective way for capturing a substantially large amount of genetic information about a cancer. Most Next-Generation Sequencing technologies involve sequencing by synthesis, while other use sequencing by ligation. Each DNA fragment to be sequenced is actually bound to an array. DNA polymerase then adds labeled nucleotides sequentially to it. Then, a high-resolution camera captures the signal from each nucleotide, as it got integrated. The camera also takes notes on the spatial coordinates and time. Then the sequence at each spot can be inferred by a computer program/ software to generate a contiguous DNA sequence, which is termed as read. Multiple Next- Generation Sequencing technology uses different ways to capture the signal and make a read. Illumina GA/Miseq, HiSeq uses fluorescent chemistry, where as Life Technologies Ion PGM, Ion Proton uses semiconductor based chemistry to capture the signal. In nutshell, Next-Generation Sequencing has revolutionized the sequencing and cost has brought down to quite an affordable level, so that it can be implemented in clinical settings for regular diagnostic procedures in molecular pathology lab.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Tridip Chatterjee

Keywords

Next generation sequencing, Molecular diagnosis, Cancer, Personalized diagnosis, Cancer

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

agrifoodaquavet@omicsonline.com

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

clinical_biochem@omicsonline.com

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

business@omicsonline.com

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

chemicaleng_chemistry@omicsonline.com

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

environmentalsci@omicsonline.com

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

engineering@omicsonline.com

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

generalsci_healthcare@omicsonline.com

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

genetics_molbio@omicsonline.com

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

immuno_microbio@omicsonline.com

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

omics@omicsonline.com

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

materialsci@omicsonline.com

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

mathematics_physics@omicsonline.com

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

medical@omicsonline.com

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

neuro_psychology@omicsonline.com

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

pharma@omicsonline.com

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

social_politicalsci@omicsonline.com

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version