alexa Abstract | Opitimization of PHB production from Escherichia coli DH5α harboring phbCAB operon using Plackett-Burman matrix and response surface methodcentral composite design

Journal of Chemical and Pharmaceutical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Original Articles Open Access

Abstract

Fermentation process of Escherichia coli DH5α harboring phbCAB operon was optimized to achieve highest PHB productivity. Neutral pH (7.0) is optimal for PHB production of E. coli that can be applied at industrial scale as neutral and basic environment (pH ≥ 7) permit bacteria growth. In this study, Plackett – Burman experiment design was used to evaluate the effects of various physical-chemical factors on PHB yield – in which Molasses amount, fermentation time and pH were proved to affect the most. This study also utilized response surface method (RSM) and central composite design (CCD) to achieve optimal value of molasses amount (120g/l), fermentation time (48h) and pH (7.0), which yieled 3.36g/l cell dried weight (CDW) and 0.87g/l PHB products. The design model was applied in 150ml aerated fermentor and 1.5l automatic fermentor, achieved 3,3g/l CDW with 0,86g/l (PHB) and 3,4g/l CDW with 0,9g/l PHB, respectively.

To read the full article Peer-reviewed Article PDF image

Author(s): HoangDung Tran TuanLoc Le HoangThang Vo and VanHieu Huynh

Keywords

PHB: poly–β–hydroxybutyrate, fermenter, Eschirichia coli DH5α, phbCAB operon, Plackett –Burman, RSM – CCD, Plackett-Burman

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords