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Original Articles Open Access
This study applied response surface methodology and synthetic evaluation method to optimize the alkaloid content of buckwheat sprouts. Solid-liquid (1:10-1:30), ethanol concentration (60%-80%), extraction temperature (20Ã¢ÂÂ-60Ã¢ÂÂ) and extraction time (20-40 min) were considered as treatment factors in the optimization. All treatment variables shown significant effects on the alkaloid content of buckwheat sprouts (P<0.05). A second order polynomial model produced a satisfactory fitting of the experimental data considering the alkaloid content (R2 adj=0.8803, S=0.094). Considering the feasibility, the best conditions for the alkaloids content were identified as solid-liquid ratio 1:15, ethanol concentration70%, extraction temperature 45Ã¢ÂÂ and extraction time 20 min, under these conditions the alkaloids content was 1.35 mg/g. This process control study provided baseline data for developing industry of buckwheat sprouts.
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Author(s): Xiaomeng Guo and Tingjun Ma
Buckwheat, Germination, Alkaloid, Response surface methodology, Ultrasonic extraction, germination buckwheat