alexa Abstract | Partial characterization of L-asparaginase-producing by Streptomyces sp. SAH1_CWMSG isolated from Rizosphere soil in Egypt

Journal of Chemical and Pharmaceutical Research
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Abstract

Fifty Streptomyces isolates were collected and screened for their capability for L-asparaginase (L-ASNase) activity. The SAH1_CWMSG isolate was found to be a promising L-ASNase producer. The SAH1_CWMSG strain possesses phenotypic characteristics typical of Streptomyces. Further phylogenetic analysis of the 16S rRNA gene sequence confirmed (99%) similarity with Streptomyces ghanensis. The strain was named Streptomyces ghanensis SAH1_CWMSG (Gen Bank ID: KT953341).The crude enzyme showed an antifungal activity against common fungal strains. In vitro, anticancer evaluation revealed that the pure enzyme exerted its action in HepG2 and MCF-7 cancer cell lines and showed promising anticancer activity compared to doxorubicin. SAH1_CWMSG strain produced L-ASNase activity of 13 IU under submerged fermentation by shake flask. The maximal activity of 106.90 IU was obtained using ammonium sulfate as nitrogen source at 28°C and 120 h on 200 rpm. The purified enzyme has Km and Vmax of 27 mMol and 69 IU/mL, respectively, with molecular weight of 38 kDa by SDS-PAGE. This is the first report on the production and characterization of L-ASNase by the SAH1_CWMSG strain. These results provided additional information on L-ASNase production and its application as an anticancer agent

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Author(s): ElSayed E Mostafa Azza M Noor ElDeen Hassan M Awad and Mamdouh A Mawad

Keywords

Streptomyces ghanensis SAH1_CWMSG, Antimicrobial and anticancer activity, Phenotypic and phylogenic identification, Asparaginase activity, characterization, production, L-asparaginase

 
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