alexa Abstract | Partial Purification and Characterization of Extracellular Protease of Proteus vulgaris and its Inhibition by the Volatile Oil of Syzygium samarangense

Asian Journal of Biomedical and Pharmaceutical Sciences
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Abstract

Background: The spread of drug resistant pathogens is one of the most serious threats to successful treatment of microbial infections. However, in the recent time, unrelenting effort to explore the medicinal importance of plants has become a major concern to scientists because of the less toxic and reliable phytopharmacoactive components of these plants. Purpose: The present study was carried out to evaluate the growth inhibitory effect of the volatile oil of Syzygium samarangense leaves against Proteus vulgaris and to examine the mode of inhibition of this oil on partially purified extracellular protease of this pathogen. Procedures: The volatile oil was extracted by hydrodistillation from air-dried leaves of Syzygium samarangense. The inhibitory effect of the oil was tested against the growth of Proteus vulgaris under favourable conditions using spectrophotometric method in nutrient broth. Similarly, the mode of inhibition against partially purified and characterized extracellular protease of this nosocomial opportunistic enteric pathogen was determined from Lineweaver Burke plot. The activity of this protease was also assessed based on the effect of different chloride salt solutions. Findings: The volatile oil of Syzygium samarangense inhibited Proteus vulgaris with IC50 of 0.42%v/v. The enzyme had optimum activity at pH 7.5 and 45°C. This enzyme was relatively stable at pH range of 7.0 - 8.0. The activity of this enzyme was moderately activated by K+ but most inhibited by Co2+. Double reciprocal plot of this enzyme showed a competitive inhibition by the volatile oil with Vmax of 8.33 x 103μmol/min and the K m in the absence and presence of volatile oil were 0.23mg/ml and 1.25mg/ml respectively. Highest percentage yield was 35.9 while the highest purification fold was 4.49. Conclusion: This study has shown that the relative stability of the protease between pH 7.0 – 8.0 confirmed one of the reasons why this pathogen is responsible for most of the urinary tract infections. The volatile oil extracted from the leaves of Syzygium samarangense possessed antimicrobial activity and its inhibition on the extracellular protease of Proteus vulgaris may just be one of its numerous modes of antibacterial activity.

To read the full article Peer-reviewed Article PDF image

Author(s): Adeola S Adesegun Folorunso O Samuel Okedeyi O Olumuyiwa Ogungbe B Folasade and Gbeliho O Grace

Keywords

volatile oil, Syzygium samarangense, antibacterial, extracellular protease, Proteus vulgaris

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords