alexa Abstract | Phosphodiesitrase activity of some Egyptian snake venoms: biochemical and immunological characteristics and effect on blood coagulation of phosphodiesterase enzyme from Naja nigricollis venom

Journal of Chemical and Pharmaceutical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Original Articles Open Access

Abstract

Phosphodiesterase (PDE) enzyme activity was screened in four Egyptian snake venoms belonging to families Elapidae and Viperadae and a highly active enzyme (Nn-PDEII), from the richest snake venom species Naja nigricollis, was purified and characterized in a set of biochemical, immunological and biological assays. Purification was followed in two successive steps including gel filtration and ion exchange chromatography. The enzyme exists as a dimer with a disulphide bridge between the two subunits. The enzyme showed its optimum activity at 60 oC and pH 8 and was best stable in alkaline medium (pH 8-9) at 40 oC up to 1 h, however, it loses ~50% of its activity at 60 oC. PDE activity was markedly increased by Mn+2, Co+2 and Mg2+ , increased to a lesser extent by Ca2+, Ba2+, Ni2+, K+ and Na+ but not significantly affected by Zn2+ ions and iodoacetic acid. On the other hand, Al3+ and Cu2+ ions, PMSF, DTT, EDTA, O-phenanthroline and L-cysteine exhibited obvious inhibitory effects. Nn- PDEII was highly immunogenic to rabbits and could induce antibodies specific to N. nigricollis venom antigens. In addition, anti-PDEII antibodies were able to partially neutralize, in vitro, PDE activity of the enzyme in a dosedependent manner. Nn-PDEII exhibited potent anticoagulant effect most probably via inhibiting one or more of the coagulation factors within the intrinsic pathway in the coagulation cascade. Characterization of a component of N. nigricollis venom that affect blood coagulation at specific stage could have potential in identifying new drug leads in the treatment of hemostatic disorders.

To read the full article Peer-reviewed Article PDF image

Author(s): N M Ibrahim W H Salama and A E El Hakim

Keywords

Phosphodiesrerase, Naja nigricollis, purification, Biochemical, Immunological, blood coagulation, snake venom

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us