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Original Articles Open Access
The leaves extracts of Lawsonia inermis, a member of the family Lythraceae, have been extracted by maceration technique in various solvents such as ethanol, ethyl acetate and n-hexane, evaluated for phytochemicals and their antibacterial potential against selected Gram negative (Proteus mirabilis and Pseudomonas aeruginosa) and Gram positive (Staphylococcus epidermidis and Enterococcus faecalis) bacterial isolates. The antibacterial activity was assessed through disc diffusion assay. It has been observed that the extracts of L. inermis leaves, have alkaloids, steroids, flavonoids and terpenoids, and saponins found in ethanol extract only. Glycosides were found in ethyl acetate and n-hexane extracts to a significant extent but glycosides were not recognized in ethanol extract. Maximum antibacterial efficacy was observed in ethanol extract and is most effective and ethyl acetate & n-hexane extracts show considerable antibacterial effect. This study shows that L. inermis leaves contain bioactive phytochemicals that may served as a biocompatible and eco-safe antiseptic or antibacterial agent in the drug formulations.
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Author(s): Mohd Yusuf
Lawsonia inermis, extraction, disc-diffusion assay, antibacterial activity, antibacterial