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Original Articles Open Access
Eichhornia crassipes (Mart.) Solms was chemically and biologically evaluated. From the phytochemical study were isolated the mix β-sitosterol and stigmasterol, 2-hydroxy-8-(4-hydroxyphenyl)-1H-phenalen-1-one and shikimic acid that were spectroscopically identified by NMR (1D and 2D). Tests on the antioxidant activities by 1,1-diphenyl-2- picryl-hydrazyl , total phenolic contents by Folin-Ciocalteau, antimicrobial activities against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Candida albicans using paper disc diffusion biossay and toxicity Brine Shrimp Lethality (BSL) were carried out for the HEX, CH2Cl2, EtOAc and n- BuOH extract. The extract EtOAc demonstrated better antioxidant capacity (EC50 66.46 ± 0.32 μg/mL) followed by extract CH2Cl2 (EC50 107.28 ± 1.43 μg/mL), and the total phenolic contents was 64.12 and 66.97 (mg EAT/mL), respectively. All extracts were inactive against the tested microorganisms. In the Brine Shrimp Lethality test, the active extract was the CH2Cl2 with DL50 49.40 ppm.
Antimicrobial, Antioxidant, Eichhornia crassipes, Phenolic content, Phytochemical study, biological investigations