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Research Article Open Access
In the present investigation different parts of Ficus racemosa L. were screened for their in vitro antimicrobial activity by well diffusion method and phytochemical screening by different conventional methods. The hydro-alcoholic extracts (50 % v/v) were prepared according to cold percolation method. The extracts were assayed against different pathogenic microorganisms. The hydro-alcoholic extracts of different parts of the tree showed maximum activity against bacterial strains in comparison to fungal strains. It was found that hydro-alcoholic extracts of leaves of the tree showed maximum potency against Lactococcus sp. at MIC value 0.5 mg/ml, but showed almost similar activity against E.coli and Aspergillus niger. The leaves extract showed no potency against Klebsiella pneumoniae, Streptococcus pyogenes and Saccharomyces cerevisae. The hydro-alcoholic extracts of the bark also showed potent activity against Lactococcus sp. at MIC value 0.3 mg/ml and showed minimum activity against Candida albicans. The extracts also showed no antimicrobial activity against E.coli, Klebsiella pnemoniae, Streptococcus pyogenes, Aspergillus niger and Saccharomyces cerevisae. The hydro-alcoholic extracts of the fruits of the plant showed maximum potency against E.coli at MIC value 0.6 mg/ml while moderate activity against Lactococcus sp. and minimum activity against Aspergillus niger. The extracts of the fruit showed no activity against Klebsiella pnemoniae, Streptococcus pyogenes, Candida albicans and Saccharomyces cerevisae. The extracts of different parts of the tree were further assayed for phytochemical screening and cellular toxicity. The extracts of different parts of the tree showed no cellular toxicity against sheep fresh blood erythrocytes.
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Author(s): Abhishek Mathur Gautam K Singh Satish K Verma Sajad Yousuf Aprajita Bhardwaj Santosh K Singh GBKS Prasad and VK Dua
Ficus racemosa L., antimicrobial activity, phytochemical screening, pathogens