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Original Articles Open Access
In present study, phytochemical screening, total phenolic content, and antimicrobial activity were carried out. Extraction was performed successively with petroleum ether, diethyl ether, acetone and distilled water. Phytochemical screening indicates the presence of various phytoconstituents. Petroleum ether and diethyl ether extracts are rich in triterpenoids while acetone extract has carbohydrate, inulin, flavonoids and phenolic compounds. Diethyl ether extract has highest concentration of total phenol (48.823%) which is responsible for its excellent antioxidant activity. Antimicrobial activity was performed against four bacterial and two fungal strains by agar well diffusion method which showed that diethyl ether extract is more prominent against all strain. These results revealed that diethyl ether extract could be used as an antimicrobial agent of natural origin in pharmaceutical industry.
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Author(s): Rishi Kumar Shukla Anirudh Porval Abha Shukla Deepak Painuly Jashbir Singh Vineet Kumar Rakesh Bhutiani and Swati Vats
Ocimum gratissimum, Phytochemical screening, Total phenolic content, Antimicrobial activity, antimicrobial activity