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Original Articles Open Access
Present study deals with enhanced saccharification of paddy straw by optimizing various physical and chemical pretreatments such as steam explosion, temperature and treatment with hydrogen chloride, a highly electronegative acid. The amount of breakdown of lignin, hemi-cellulose & cellulose was determined using Fourier Transform Infrared Spectroscopy (FTIR). The maximum amount of breakdown of lignin is observed for 90minutes duration at wave number 1462.88cm-1 and maximum exposure of xylan is observed at wave number 1602.9cm-1for 90 minutes exposure to steam explosion. In coupled pretreatment method (steam explosion & acidic simultaneously given) maximum cellulose breakdown took place after acidic pretreatment followed by steam explosion which was observed by FTIR at 1320.32cm-1 wave number simultaneously histological changes were determined by staining and visualization at 10X using saffranin dye. In the qualitative analysis using High Pressure Liquid Chromatography of sample after 90 minutes steam explosion chromatogram of Xylitol (Rt = 9.886) & 60 minutes acidic pretreatment chromatogram of, Xylose (Rt = 8.741) were observed.
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Author(s): Vipul Swarup Bhatnagar Neeta Raj Sharma and Sachin Kumar
lignocellulosic, optimization, saccharification, steam explosion