alexa Abstract | Production podophyllotoxin by root culture of Podophyllum hexandrum Royle

Electronic Journal of Biology
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Abstract

A way of production podophyllotoxin by root culture of Podophyllum hexandrum Royle (P. hexandrum) was established. In the basis of aseptic plantlets induced from mature embryo of P. hexandrum seed, the rhizome of the plantlets were excised to 2.0 cm and then cultured on the Murashige and Skooge medium (MS) supplemented with different concentrations of growth regulators (IBA: 3-Indolebutyric acid, GA3: Gibberellic acid, Hy: Hydroquinone) and activated charcoal (AC). As the cultures development, the Hy could promote the plantlets growth compared to IBA and GA3, and the length of rhizome reached by 11.59 cm under the concentration of Hy 2.0 mg/L after 40 days; Supplementation of the medium with AC could significantly hasten the plantlet growth and development compared to other treatments with the length and dry weight of rhizome 13.60 cm and 0.33 g under the concentrations of Hy 2.0 mg/L and AC 0.5 g/L after 40 days; MS + sucrose 30.0 g/L + agar 5.0 g/L + Hy 5.0 mg/L + AC 0.5 mg/L (pH=5.8) was optimum for both biomass and accumulation of podophyllotoxin in root culture of P. hexandrum, the content of podophyllotoxin in the root and cultured medium reached 151.50 μg/g and 121.22 μg/g with the accumulation index of podophyllotoxin e0.4487 and e0.3329 respectively after 50 days. This indicated that production podophyllotoxin by root culture of P. hexandrum was feasible and had important application prospect.

To read the full article Peer-reviewed Article PDF image

Author(s): Wei Li Mengfei Li Delong Yang Rui Xu Yingru Zhang

Keywords

Podophyllum hexandrum Royle, Growth regulators, Activated carbon, Root culture, Podophyllotoxin.

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords