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Original Articles Open Access
The current study presents a simple, rapid, precise technique for the quantification of Ornidazole from bulk and pharmaceutical formulations. The analysis was carried out using a C18 column (250 mm ´ 4.6 mm; 5 μm) using a combination of 0.53 mM phosphate buffer and acetonitrile in mobile phase at a flow rate of 1ml per minute. The chromatogram presents curves free from co-elution at a detection wavelength of 305nm. The method has been validated as per ICH Q2 guidelines. The method was found to linear within concentration range 10.49μg/ml and 83.96μg/ml with limit of detection and limit of quantitation 0.17μg/ml and 0.52μg/ml respectively. The method was found to be suitable for the regular analysis of ornidazole form marketed formulations.
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Author(s): Amit Kumar De Ashok Kumar Bera and Biswajit Pal
Liquid Chromatography, Ornidazole, Validation., chromatographic technique