700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ ReadersThis Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
A simple and rapid real-time reverse transcription-PCR (RT-PCR) technique using SYBR green fluorescence was developed to quantify Zonula Occludens-1 (ZO-1) mRNA expression in histamine-treated cultured bovine retinal microvessel endothelial cells. There was no dif-ference between the quantity of ZO-1 mRNA expression in histamine-treated (10-7M to 10-9 M) and untreated bovine retinal endothelial cells at high magnesium levels. Immunohisto-chemistry staining and western blot assay unequivocally demonstrated the approximately similar levels of ZO-1 protein in both histamine-treated and non-treated endothelial cells at high magnesium concentrations.
To read the full article Peer-reviewed Article PDF
| Peer-reviewed Full Article
Author(s): Sekaran Muniandy Koon Chu Yaiw Jack Bee Chook Arokiasamy Vinscent Rayappan Kum Thong Wong Ikram Shah Ismail and Rajes Qvist
Diabetes, Bovine retinal endothelial cells, Zonula Occludens-1, Real time reverse transcription poly-merase chain reaction