alexa Abstract | Quantitative determination of active constituent in fructus trichosanthis and its anti-proliferative effect on cervical carcinoma HeLa cells

Biomedical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access

Abstract

To establish a method for determination of quercetin content in fructus trichosanthis, and to observe the inhibitory effect of fructus trichosanthis extract on growth of cervical carcinoma HeLa cells. Active constituent was determined by HPLC with quercetin as the reference using 0.3% phosphoric acid: methanol solution (47:53) as the mobile phase at a detection wavelength of 360 nm. MTT assay was used to determine the cell metabolic rate, while flow cytometry was utilized to observe the changes in apoptosis. Quercetin showed good linearity within a 1.0468-10.468 mg/mL range (r=0.9997), with an average recovery of 98.58%. Compared to the control group, different concentrations of fructus trichosanthis extracts all inhibited HeLa cell proliferation after acting on for 24 and 48 h. Moreover, inhibition rates increased significantly with increasing drug concentrations and prolonging treatment time, showing a certain time-dose-response relationship. S phase ratio of HeLa cells rose from 24.68% in the control group to 42.15%, while G2 phase cell ratio dropped from 10.54% in the control group to 5.11%. Fructus trichosanthis extract could arrest HeLa cells in S phase. Compared to the control group, percentage of S phase cells increased, while G2 phase cells decreased. And such effect was dosedependent. The present quantitative determination method is reproducible, accurate and reliable. Fructus trichosanthis extract has a significant inhibitory effect on the growth of HeLa cells in vitro.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): CaiHong Zhang QianQian Sun JingJing Hu

Keywords

Fructus trichosanthis, Quercetin, Content determination, MTT, Flow cytometry, #

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords