alexa Abstract | Quantitative determination of Radix ophiopogonison and its protective effect on intestinal ischemia reperfusion-induced lung injury in rats.

Biomedical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)


To establish a RP-HPLC method for the determination of ophiopogonin A content in Radix ophiopogonison; and to observe the intervention effect of Radix ophiopogonison extract on acute lung injury after intestinal ischemia reperfusion. Synergi 4 μ Hydro-RP 80A column (250 mm × 4.6 mm, 5 μm) is used, column temperature is 25°C, mobile phase is water-acetonitrile (volume ratio 62:38), flow rate is 1.0 mL/min and detection wavelength 208 nm. Rat model of intestinal ischemia reperfusion is established, and then the rats are randomized into control group, ischemia reperfusion group and Radix ophiopogonison intervention group. Total protein content in Bronchoalveolar Lavage Fluid (BALF), dry/wet weight ratio (D/W) of lung tissues, peroxidase (MPO) content in lung tissues and Malondialdehyde (MDA) content in plasma are measured. Mass concentration of ophiopogonin A shows a good linear relationship (r=0.9999) with the peak area at a range of 0.020~0.200 mg/mL, and the average recovery is 99.75% (RSD=0.22%, n=9). Compared with the ischemic reperfusion group, BALF total protein content increases significantly, while lung D/W decreases significantly in the intervention group. The content determination method established herein is simple and accurate, which can be used for the quality control of Radix ophiopogonison medicinal material. Radix ophiopogonison extract can significantly reduce the degree of acute lung injury.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Wenjie Fan Xiaohui Sun Yong Sun Xianmin Jiang


Radix ophiopogonison, Ophiopogonin A, Ischemia reperfusion, Intestinal ischemia reperfusion, Lung protection, #

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version