alexa Abstract | Quantitative estimation of Rutin from leaves of in vivo and in vitro plants of Ruta graveolens by HPTLC method

Asian Journal of Plant Science & Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access

Abstract

Ruta graveolens L., commonly known as Garden rue is member of Rutaceae, has known for its rich aromatic and medicinal properties., widely exploited for its active principles which are of high pharmaceutical value, The plant contains variety of natural compounds, One of the most important flavonoid in R. graveolens L.is Rutin. It has shown a wide range of biological activities. Therefore, efforts have been taken to estimate amount of Rutin from in vivo and in vitro plants of R. graveolens.The powdered leaves were extracted with methanol in a Soxhlet extractor. Standard Rutin and samples were applied to the plates as 8 mm bands on the silica gel HPTLC plate (60 F 254, E. Merck, Germany, 10×10 cm, 0.2 mm thick) as 10 mm wide bands with TLC applicator Linomat 5 with inert gas flow (Camag, Switzerland), The HPTLC plates were developed with developing solvent, Ethyl acetate: Formic acid: Water (8:1:1). Quantitative evaluations of the plates were performed in the reflectance/absorbance mode at 362 nm. The amount of Rutin was estimated by the comparing the peak area of standard Rutin.



Author(s): Ajit A Kengar and Govind T Paratkar

Keywords

Rutin, HPTLC and Ruta graveolens, Plant Biotechnology

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords