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Short Communication Open Access
A simple, economical, fast and precise reverse phase high performance liquid chromatographic method has been developed for the simultaneous determination of zidovudine and lamivudine in from tablet dosage form. A BDS Hypersil C18 (5 micron 25 cmx4.6 mm) column from Thermo in isocratic mode with mobile phase o-phosphoric acid:methanol (70:30) buffered and adjusted to pH 5 by using triethylamine. The flow rate is 1.4 ml/min and effluent is monitored at 220 nm.
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Author(s): M S Palled P M N Rajesh M Chatter A R Bhat