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Short Communication Open Access
A reversed-phase HPLC (RP/HPLC) method using Lichrosphere RP select B, C8 column has been developed for the simultaneous determination of ipratropium bromide, methylparaben and propylparaben in formulations. The mobile phase consists of an aqueous solution containing heptane sulphonic acid sodium salt (pH 3.2) and 345 ml of acetonitrile, with detection at 210 nm. Recovery study values of 98-102% and a relative standard deviation of less than 1% for the assay showed that the method is accurate and precise.
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Author(s): K R Premnath Sheony K S Krishnamurthy Vasudhara Iyengar J Harsha