alexa Abstract | RP-HPLC and HPTLC methods for the estimation of carvedilol in bulk drug and pharmaceutical formulations

Indian Journal of Pharmaceutical Sciences
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Short Communication Open Access

Abstract

Two simple, specific, accurate and precise methods, namely, reverse phase high performance liquid chromatography and high performance thin layer chromatography were developed for estimation of carvedilol in bulk drug and pharmaceutical formulations. For the high performance liquid chromatography method, Lichrospher 100 C-18, 5 µm column consisting of 200×4.6 mm i.d. in isocratic mode, with mobile phase containing 50 mM KH2PO4 buffer (pH 3.0±0.1): acetonitrile: methanol (60:50:10 v/v/v) was used. The flow rate was 1.0 ml/min and effluent was monitored at 242 nm. The retention time was 4.56±0.03 min. For the high performance thin layer chromatography method a Camag high performance thin layer chromatography system comprising of Linnomat V automatic sample applicator, Hamilton Syringe, Camag TLC Scanner-3, Camag Win CAT software with stationary phase precoated silica gel 60F254 and mobile phase consisting of ethyl acetate: toluene: methanol (1:4:3.5 v/v/v). The detection of spot was carried out at 242 nm. The Rf value was 0.65±0.02. The methods were validated in terms of linearity, accuracy and precision. The linearity curves were found to be linear over 1-35 µg/ml for high performance liquid chromatography and 50-300 ng/spot for high performance thin layer chromatography. The limit of detection and limit of quantification for high performance liquid chromatography were found to be 0.2 and 0.85 µg/ml, respectively, and for high performance thin layer chromatography, 10 and 35 ng/spot, respectively. The proposed methods were successfully used to determine the drug content of marketed formulations.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): LJ Patel BN Suhagia PB Shah RR Shah

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

generalsci[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords