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A high-performance liquid chromatographic method for the determination of Torsemide is described. The assay uses a reversed-phase gradient system and UV-detection. The chromatographic separation was carried out on a μBondapak C18 column with a mobile phase consisting of Acetonitrile/Phosphate Buffer 0.05M (pH 2.4) in ratio of 70/30. The method was validated and found to be linear in the range of 50-100 μg/ml. The retention time of Torsemide was 6.00 ± 0.20 min. The chromatograms showed good resolution and no interference with impurity. The mean recovery of the Torsemide was found to be above 99.9%. Both accuracy and precision data showed good reproducibility. The linearity range was found to be 50-100 μg/ml with coefficient of variation of 0.998 at calibration point. The limit of detection and limit of quantization for Torsemide were found to be 148.1ng and 448.9ng respectively. These results suggested that the analytical method was linear, precise, and accurate.
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Author(s): Rajesh Shukla Nidhi Bhavsar Vikas Pandey Dilip Golhani Alok Pal Jain
HPLC, Acetonitrile, isocratic, Torsemide