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Short Communication Open Access
A simple, efficient and reproducible method for the simultaneous determination of gatifloxacin and ornidazole from tablets has been developed using reversed phase high performance liquid chromatography. The separation was done using a mobile phase consisting of acetonitrile: methanol: water (50:37.5:12.5). Column used was Hypersil C18 (250×4 mm i.d.) 5 m with a flow rate of 1 ml/min with detection at 280 nm. The external standard calibration method was employed for quantification. An elution order of gatifloxacin was 2.58 min and ornidazole was 5.54 min. The linear dynamic range was 20-100 mg/ml and 50-250 mg/ml for gatifloxacin and ornidazole, respectively. Analytical parameters were calculated and a full statistical evaluation included.