alexa Abstract | RP-HPLC method for the estimation of Rifabutin in bulk dosage form

International Journal of Drug Development and Research
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Abstract

A simple, economic, accurate and precise reverse phase high performance liquid chromatographic method was developed and validated for the determination of rifabutin. A phenomenex C-8 Luna (250 × 4.6 mm, 5 μm) in isocratic mode with mobile phase containing Methanol and Water (75:25 v/v) was used. The flow rate was 1 ml/min and effluents were monitored at 240 nm and run time was 10 min. The retention time for Rifabutin was 5.5 min. The RP-HPLC method was extensively validated for linearity, accuracy, precision (Interday precision, Intraday precision), repeatability and robustness. The validation studies were carried out fulfilling ICH requirements. The procedure was found to be linear, precise, accurate and robust

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Author(s): Yogesh D Patil and Saurabh K Banerjee

Keywords

Rifabutin, RP-HPLC, Phenomenex, Methanol

 
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