700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ ReadersThis Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Original Articles Open Access
A simple, rapid, and accurate reversed phase ultra-performance liquid chromatographic (RP-UPLC) method has been developed and subsequently validated for the simultaneous determination of proguanil (PRG) and Atovaquone (ATV) in combination. The separation is carried out using a mobile phase consisting of 0.05% perchloric acid and Acetonitrile. The column used is Extended C18(50 × 3.0 mm, 1.8 μm). With flow rate of 0.8 mL/min using UV detection at 257nm. The total run time is 6 min and the retention time of PRG and ATV is 1.6 minand 3.0 min respectively. The described method is linear for the assay of PRG and ATV over a concentration range of 2.5-20 μg/Ml and 6.25-50 μg/mL respectively. Results of the analysis have been validated statistically and by recovery studies. The limit of quantitation for PRG and ATV has been found to be 0.0357 μg/mL and 0.126 μg/mL respectively. The results of the studies showed that the proposed RP-UPLC method is simple, rapid, precise, and accurate, which is useful for the routine determination of PRG and ATV in bulk drug and its pharmaceutical dosage form.
To read the full article Peer-reviewed Article PDF
Author(s): Khagga BhavyasriV Murali Balaram R Nageswarao D Rambabu and M Ajitha
Atovaquone, proguanil, UPLC, Simultaneous and Validation, pharmaceutical dosage