alexa Abstract | Simultaneous determination and validation of gallic acid and quercetin in Anisomeles malabarica R. Br. Ex Sims using high performance thin layer chromatography

Journal of Chemical and Pharmaceutical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Original Articles Open Access


To analyse the two bioactive components Gallic acid and Quercetin from the methanolic extract of aerial part of Anisomeles malabarica R.Br. Ex Sims. Chromatography Separation was carried out on aluminium plate precoated with silica gel 60F254 with toluene: ethyl acetate: formic acid (5.0:2.5:0.5, v/v/v) as solvent system. Densitometry scanning was performed under reflectance- absorbance mode at 366 nm to quantify the spots. The Rf values of Gallic acid and Quercetin were found to be 0.24 and 0.40 respectively. Linearity of r2 = 0.999 and 0.998, LOD 0.6 and 0.7 μg/band, LOQ 0.7 and 0.8 μg/band and recovery of 97.62% and 97.55% respectively were satisfactory for Gallic acid and Quercetin. The HPTLC method was validated as per the ICH guideline. The developed simple, precise, specific, sensitive and accurate method can be used for routine quality control of raw material of Anisomeles malabarica R.Br. Ex Sims as well as formulation containing any of these bioactive compounds.

To read the full article Peer-reviewed Article PDF image

Author(s): Sheikh Mohmad Vasim Devadiga Navin and Hate Manish


HPTLC, Anisomeles malabarica, Gallic acid and Quercetin, gallic acid

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version