alexa Abstract | Simultaneous determination of Atorvastatin, Glimepiride and Amlodipine in solution and plasma matrix using HPLC/UV method

Journal of Chemical and Pharmaceutical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Original Articles Open Access

Abstract

The aim was to develop and validate a simple, fast, reliable, selective and accurate HPLC method with UV detection for simultaneous determination of atorvastatin, glimepiride and amlodipine in a solution and plasma matrix. The method consisted of a mobile phase containing water, methanol and acetonitrile at 1.58:1:1(v/v%) buffered with triethylamine at pH8.0, a flow rate of 1.5 ml/min and a UV detector at 237 nm wavelength. Different and slight variations were introduced in the mobile phase, pH, wavelength, and column temperature to ensure method robustness in yielding good accuracy and precision. Other parameters such as sunlight sample exposure and acid degradation were evaluated on solutions containing the three drugs. A successful HPLC method was validated and developed to detect and quantify atorvastatin, glimepiride and amlodipine in a solution and plasma matrix, system and method precision were reasonable as RSD% values were below 2%, calibration curves revealed linearity in the range of 0.5-1.5 μg/ml for all the three drugs dissolved in the diluent with R2>0.99. RSD% in the linear range and for each of the calibration curve is between 0.03-0.32 for amlodipine, 0.03-0.36 for glimepiride and 0.18-0.46 for atorvastatin. Accuracy was with %recovery for each drug concentration between 98-102% which was considered as acceptable according to USP guidelines for analytical method validation. Stability tests represented reasonable stability of method for three drugs in both plasma and solutions as the % recovery was between 98-102%. Robustness was reliable as wavelength variation,pH variation, acid /base degradation, temperature changes and acetonitrile change in mobile phase showed RSD% less than 2% for all. The method was selective for the three drugs without any possible interference between excipients present in a tablet and the three drugs. Results indicated the method suitability to be used for determination of amlodipine, glimepiride and atorvastatin simultaneously in two different matrices, diluent and plasma.



Author(s): Wael Abu Dayyih Alaa Alfayez Lina Tamimi Eyad Mallah and Tawfiq Arafat

Keywords

Atorvastatin, Glimepiride, Amlodipine, HPLC, Solution, Plasma., Atorvastatin

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords