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A new simple, rapid and sensitive HPLC method has been developed and validated for the simultaneous determination of chlorpheniramine maleate, paracetamol and phenylephrine hydrochloride in pharmaceutical preparation. Separation was achieved on a 250mm x 4.6mm, 5μm particle, C8 column using an isocratic mobile phase consisting of 0.01M phosphate buffer: acetonitrile (70:30), pH of the mobile phase was adjusted at 3 with 50% orthophosphoric acid. The analysis was performed at flow rate of 1mLmin-1. Detection of all compounds was carried by UV absorbance at 230nm and elution of the analytes was achieved in less than 8 minutes. The method was validated as per ICH guidelines. The linearity, accuracy and precision of the method were acceptable to good over the concentration range of 5- 60μg mL-1 for all the three drugs. The lower limit of detection was found to be 0.36, 0.36 and 0.28μg mL-1 for chlorpheniramine maleate, paracetamol and phenylephrine hydrochloride respectively. The developed method is effectively used for the routine analysis of formulations for both quality control and assessment of stability.
Reversed phase HPLC, Paracetamol, Chlorppheniramine maleate, Phenylephrine hydrochloride, Simultaneous estimation