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The objective of this work was to develop and validate a high performance thin layer chromatography method for simultaneous determination of Guggulosterone-Z and Diosgenin in combined dosage form. The stationary phase used was silica gel G60F254. The mobile phase used was mixture of ethyl acetate: methanol: water (4:1:0.5 v/v/v). The detection of spots was carried out at 254 nm using absorbance reflectance mode. The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 200 to 800 ng/spot for guggulosterone Z and 50 to 350 ng/spot for diosgenin. The limit of detection and limit of quantification for the Guggulosterone-Z were found to be 5.05 and 15.08 ng/spot,respectively and for diosgenin 9.23 and 24.15 ng/ spot, respectively. The proposed method can be successfully used to determine the drug content of marketed formulation.
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Author(s): Nitin Mogal Mohan Agrawal
Diosgenin, HPTLC, simultaneous estimation, Guggulosterone-Z