alexa Abstract | Simultaneous Determination of Some Antihypertension Drugs in Their Binary Mixtures by Simple Spectrophotometric Methods

Asian Journal of Biomedical and Pharmaceutical Sciences
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Abstract

Two simple, accurate and cost effective dual wavelength (Method I) and mean centering of ratio spectra MCR (Method II) spectrophotometric methods are developed and validated for determination of two drug combinations. The first comprises Hydrochlorothiazide (HCZ) and Benazepril Hydrochloride (BZ) and the second comprises HCZ and Candesartan Cilexetil (CAN) in their bulk powder and pharmaceutical tablets. In method (I), two wavelengths were selected for each drug in such a way that the difference in absorbance was zero for the second drug. For BZ/HCZ, mixture (I), BZ had equal absorbance values at 224.2 and 252.9 nm, therefore these two wavelengths were used to determine HCZ. Similarly, 243.8 and 293.8 nm were selected to determine BZ, where HCZ had equal absorbance values. For CAN/HCZ, mixture (II), HCZ is determined at 242.2 and 264.4 nm while CAN is determined at 257.8 and 280 nm. In method (II), absorption spectra of each drug were recorded, divided by suitable divisor and the obtained ratio spectra were mean centered. The concentrations of the proposed drugs were then determined from the calibration curves obtained by measuring amplitudes at 274 and 240.2 nm for HCZ and BZ respectively for mixture (I) and 274 and 240.4 nm for HCZ and CAN respectively for mixture (II). The developed methods were validated according to ICH guidelines demonstrating good accuracy and precision. The results were statistically compared with those obtained by reported methods indicting no significant difference and ability of methods to be used for routine analysis of proposed drugs.

To read the full article Peer-reviewed Article PDF image

Author(s): Eglal A Abdelaleema Ibrahim A Naguiba Hala E Zaazaab and Mohammed E Drazc

Keywords

Hydrochlorothiazide, Benazepril Hydochloride, Candesartn Cilexetil, Dual wavelength, Mean centering of ratio spectra, spectrophotometry.

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us