alexa Abstract | Simultaneous determination of two biflavones in Biyanling Tablets by HPLC.

Biomedical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)


Objective of the present study is to develop analytical methods for simultaneous determination of two major biflavones, amentoflavone and heveaflavone, in Biyanling Tablets, thereby providing methodological reference for quality control of anticancer drug Biyanling Tablets. Samples are chromatographed on Diamonsil C18 (4.6 mm × 250 mm, 5 μm) column with a mobile phase of acetonitrile (B)–0.5% acetic acid solution (A); other chromatographic conditions are as follows: elution gradient 0~4 min, B: 35%→45%; 4~20 min, B: 45%→50%, detection wavelength 270 nm, flow rate 1.0 mL/min, and column temperature 30?. Amentoflavone and heveaflavone show good linear relationships (r≥0.9998 and 0.9996) within a range of 1.56~100 μg/mL, average recoveries are 98.2% and 96.5%, with RSDs of 2.1% and 2.4 % (n = 6), respectively. The method is simple, fast and reproducible, which provides a quantitative analytical method for quality control of Biyanling Tablets.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Yang Yang Xinye Tang Hongbing Yao


Biyanling, biflavone, content determination, HPLC

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us