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Research Paper Open Access
A simple, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of bisoprolol fumarate and hydrochlorothiazide in tablet dosage form. A lichrospher 100 C-18, 5 µm column 20 cm × 4.6 mm in isocratic mode, with mobile phase containing water, acetonitrile and tetrahydrofuran in proportion of 80:20:5 v/v/v were used. The flow rate was 1 ml/min, and effluent was monitored at 225 nm. The retention time of bisoprolol fumarate and hydrochlorothiazide were 1.48 ± 0.02 and 4.72 ± 0.03 min respectively, and the resolution factor was 9.0. Linearity values for bisoprolol fumarate and hydrochlorothiazide were in the range of 10-150 µg/ml and 1-90 µg/ml respectively. The limit of detection and limit of quantification for bisoprolol fumarate was found to be 3.5 and 8.5 µg/ml respectively; and for hydrochlorothiazide, 0.4 and 0.9 µg/ml respectively. The proposed method is accurate, precise, specific and rapid for simultaneous estimation of bisoprolol fumarate and hydrochlorothiazide in tablet dosage form.