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Short Communication Open Access
Three simple, rapid, accurate, economical and reproducible procedures for simultaneous estimation of gliclazide and metformin hydrochloride in two component tablet formulation have been developed. First method is based on an equation of area calculation of curve at two wavelength regions (228.6 to 224 nm and 235 to 231 nm). Second method employs simultaneous equation at two wavelengths corresponding to 226.3 and 233.2 nm. Third method employs derivative spectroscopy at zero crossing points (226 and 232.9 nm) in first order derivative spectra. Both the drugs obey Beer's law in the concentration ranges employed for these methods. The results of analysis have been validated statistically and by recovery studies.
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Author(s): H K Jain R K Agrawal