alexa Abstract | Simultaneous estimation of Metformin, Pioglitazone and Glimepiride in bulk samples and in tablet dosage forms by using RP-HPLC in an Isocratic mode

Journal of Chemical and Pharmaceutical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Original Articles Open Access

Abstract

The present work was undertaken with the aim to develop and validate a rapid and consistent RP-HPLC method in which the peaks will be appear with short period of time as per ICH guidelines. The HPLC separation was achieved on an XTerra C18 column in an Isocratic Mode. The mobile phase was composed of phosphate buffer (25 %) [pH 3.0 adjusted with OPA] and methanol (75 %) The flow rate was monitored at 1.0mL per min. The wavelength was selected for the detection was 254 nm. The retention time found for metformin, pioglitazone and glimepiride were 1.997, 3.238 and 4.042 min respectively. The % recovery was found 98.46- 101.79 for metformin, 98.04 - 101.79 for pioglitazone and 99.60 - 100.45 for glimepiride. The linearity was established in the range of 80-120 μg/mL for metformin and 2.4-3.6 μg/mL for pioglitazone and 0.16-0.24 μg/mL for glimepiride. The LOD for metformin, pioglitazone and glimepiride were 0.07, 0.07 and 0.006 μg/mL respectively. The LOQ for metformin, pioglitazone and glimepiride were 0.23, 0.24 and 0.02 μg/mL respectively. The proposed method was adequate sensitive, reproducible, and specific for the determination of metformin, pioglitazone and glimepiride in bulk samples as well as in tablet dosage form.

To read the full article Peer-reviewed Article PDF image

Author(s): S Ashutosh Kumar Manidipa Debnath J V L N Seshagiri Rao and D Gowri Sankar

Keywords

Metformin, Pioglitazone, Glimepiride, ICH Guideline, RP-HPLC, LOD, LOQ., RP-HPLC

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords