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Short Communication Open Access
The present work describes a simple reverse phase HPLC method for the determination of levonorgestrel and ethinylestradiol from tablet formulations. The determination was carried out on a Hypersil, ODS, C-18 (150Ã—4.6mm, 5 micron) column using a mobile phase of acetonitrile/ water (42:58). The flow rate and runtime were 2.2 ml/min and 7 min, respectively. The eluent was monitored at 210 nm. The method was found to be reproducible, with good resolution between levonorgestrel and ethinylestradiol. The detector response was found to be linear in the concentration range of 10-50 ppm for levonorgestrel and 2-10 ppm for ethinylestradiol.
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Author(s): S Durga Prasad G Changala Reddy P S S Prasad K Mukkanti