alexa Abstract | Simultaneous HPTLC Determination Of Rifampicin And Isoniazid In Rat Plasma

Indian Journal of Pharmaceutical Sciences
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Short Communication Open Access


A method enabling the precise and quick simultaneous assessment of rifampicin and isoniazid in rat plasma by HPTLC has been presented. Extracted samples of rifampicin and isoniazid in organic solvents were separated on a plate coated with silica gel 60 F254, chromatograms were developed using a mixture of chloroform:methanol and quantification was carried out by the use of densitometer absorbance mode at 475 nm and 280 nm respectively. Under the operating conditions, the lower limit of detection was found to be 0.3 μg/spot for rifampicin and 0.1 μg/spot for isoniazid. The method has a linearity range of 10 to 80μg/ml for both the drugs and recovery was found to be 97.5% for both rifampicin and isoniazid. The pharmaiokinetic parameters obtained after intravenous bolus injection at a dose of 10 mg/kg has also been presented.

To read the full article Peer-reviewed Article PDF image

Author(s): P Goyal S Agarwal S Venkatesh S Pandey N Udupa

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us