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Short Communication Open Access
The present study provides us a single analytical tool for the determination of elvitegravir, cobicistat, emtricitabine and tenofovir disoproxil fumarate in dosage forms. This method helps us to separate and detect the four active pharmaceutical ingredients in a single run. The objective is to develop and validate a simple reverse phase liquid chromatography method for the simultaneous determination of emtricitabine, tenofovir disoproxil fumarate, elvitegravir and cobicistat in dosage tablets. For this method, an Agilent 1200, high performance liquid chromatography system, Atlantis C18 column (100×4.6 mm, 5 μm) as stationary phase and a gradient mixture of 0.1% trifluoroacetic acid and acetonitrile was used as mobile phase. The gradient program was adjusted at 1.0 ml/min flow rate and 10 μl injection volumes were maintained. The eluted compounds were monitored at 240 nm. The column oven temperature was maintained at 30°. The developed chromatographic method was validated for selectivity, linearity, precision, accuracy, sensitivity, robustness, and system suitability.
Elvitegravir, Cobicistat, Emtricitbine, Tenofovir Disoproxil Fumerate, HPLC method validation, simultaneous determination