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Short Communication Open Access
Two simple, accurate and precise methods for simultaneous estimation of pioglitazone hydrochloride and glimepiride in combined dosage form have been described. First method employs formation and solving of simultaneous equation using 268.6 nm and 227.6 nm as two analytical wavelengths. Second method is absorbance ratio method, 268.6 nm for estimation of pioglitazone hydrochloride and 251.5 nm, isobestic point, where two drugs shows equal absorptivity, for the estimation of glimepiride. Both the methods allow the simultaneous determination of pioglitazone hydrochloride and glimepiride in the concentration ranges employed for this purpose. The result of analysis has been validated statistically and by recovery studies and assay of tablets with standard deviation < 1.0% was found.
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Author(s): Shveta Chandna A V Kasture P G Yeole