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Short Communication Open Access
A simple, sensitive and reproducible spectrophotometric method was developed for the estimation of andrographolides in Andrographis paniculata ( Kalmegh ) and its formulation. The method is based on the condensation of g-unsaturated lactone ring of andrographolides and picric acid in alkaline medium which results in the formation of colored complex, that which could be measured at 494 nm. Linearity of the proposed method was found between 5-50 µg/ml. The powdered samples of A. paniculata procured from different sources were found to contain 0.9-1.5% w/w of andrographolides. Formulation containing A. paniculata showed 0.3% w/w of the andrographolides. Our studies clearly indicated that Baljet reagent can also be employed for estimation of andrographolides. The method was found to be reliable and precise and could be used for analyzing formulations containing andrographolides for regular quality control purposes.