alexa Abstract | Spectrophotometric Determination of Chloroquine in the Hair of Mice

Asian Journal of Biomedical and Pharmaceutical Sciences
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)


Background: Hair analysis for illicit, recreational and therapeutic drugs though an evolving science is easier, less invasive of privacy and there is less chance of tampering with the sample to affect results. Often, a combination of capillary gas chromatography with mass spectrometry, though with known short comings, is preferred for detection of drugs in hair specimen.
Objectives: This study aimed at determination of chloroquine in the hair of mice by Ultra-Violet spectrophotometry. It has the advantage of being easy to operate and sensitive to trace amount. Methods: Twenty-five mice weighing about 25g each were administered chloroquine phosphate intramuscularly once a week for 4 weeks. Hair was harvested from the same spot before, during administration and at the end of dosing. The samples of hair were extracted with methanol and the resultant residue dissolved in 0.1M HCl. The resulting solutions were then run with the Ultra-Violet spectrophotometer to determine their concentrations using the calibration curve of the reference material.
Results: There was proportionate significant increase in the concentration of chloroquine phosphate in hair at the intervals of administration. The amount of chloroquine detected in hair samples increased from 2.98x 10- 3/10g of hair on the second week of administration to 4.88x10-3/10g of hair on the fourth week of administration.
Conclusion: The result from the present study showed that although a new innovation, hair testing using Ultra-Violet spectrophotometry is a valuable has been seen as a valuable tool for drug analysis.

To read the full article Peer-reviewed Article PDF image

Author(s): Olufunso OS Matthew O


Ultra-Violet-Spectrophotometry, Hair, Chloroquine, Mice

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version