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Short Communication Open Access
Spectrophotometric, spectrofluorimetric and high performance liquid chromatographic methods for the determination of hexamine in pure form and in hiprex tablet are described Both hexamine and hiprex form stable yellow colored 3,5-diacetyl-1,4-dihydrolutidinae t 100o when treated with acetylacetone in phosphate buffer (pH 6.0). The chromogen shows absorption maximum at 410 nm and a fluorescence emission maximum at 510 nm. In the case of hiprex, Beer's law linearity was observed in the concentration ranges of 10-150 Î¼g/ml, 3-30.5 Î¼g/ml and 0.02-0.35 Î¼g/ml in spectrophotometric, fluorimetric and high performance liquid chromatographic determinations, respectively. The common additives usually present in the tablet did not interfere in the proposed methods.
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Author(s): M Balasubramanian S Thennarasu PT Perumal AB Mandal