alexa Abstract | Spectrophotometric, Spectrofluorimetric And High Performance Liquid Chromatographic Method For The Determination Of Hexamine In Tablets

Indian Journal of Pharmaceutical Sciences
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Spectrophotometric, spectrofluorimetric and high performance liquid chromatographic methods for the determination of hexamine in pure form and in hiprex tablet are described Both hexamine and hiprex form stable yellow colored 3,5-diacetyl-1,4-dihydrolutidinae t 100o when treated with acetylacetone in phosphate buffer (pH 6.0). The chromogen shows absorption maximum at 410 nm and a fluorescence emission maximum at 510 nm. In the case of hiprex, Beer's law linearity was observed in the concentration ranges of 10-150 μg/ml, 3-30.5 μg/ml and 0.02-0.35 μg/ml in spectrophotometric, fluorimetric and high performance liquid chromatographic determinations, respectively. The common additives usually present in the tablet did not interfere in the proposed methods.

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Author(s): M Balasubramanian S Thennarasu PT Perumal AB Mandal

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